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Mouse Aak1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Mouse Aak1 Antibody (Center) AP14604c
    Mouse Aak1 Antibody (Center) (Cat. #AP14604c) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the Aak1 antibody detected the Aak1 protein (arrow).
  • IHC-P - Mouse Aak1 Antibody (Center) AP14604c
    Mouse Aak1 Antibody (Center) (AP14604c)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Aak1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q3UHJ0
Other Accession P0C1X8, F1SPM8, Q2M2I8, F1MH24, NP_808430.2, NP_001035195.1
Reactivity Mouse
Predicted Bovine, Human, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 103346 Da
Antigen Region 279-306 aa
Additional Information
Gene ID 269774
Other Names AP2-associated protein kinase 1, Adaptor-associated kinase 1, Aak1, Kiaa1048
Target/Specificity This Mouse Aak1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 279-306 amino acids from the Central region of mouse Aak1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Aak1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Aak1
Synonyms Kiaa1048
Function Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity (By similarity).
Cellular Location Cell membrane; Peripheral membrane protein. Membrane, clathrin-coated pit. Note=Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells (By similarity).
Research Areas
Citations (0)

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Aak1 phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis (By similarity).


Munton, R.P., et al. Mol. Cell Proteomics 6(2):283-293(2007)
Nishimura, M., et al. DNA Res. 11(5):315-323(2004)
Jha, A., et al. J. Biol. Chem. 279(3):2281-2290(2004)
Zambrowicz, B.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100(24):14109-14114(2003)
Hansen, J., et al. Proc. Natl. Acad. Sci. U.S.A. 100(17):9918-9922(2003)

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Cat# AP14604c
(40 western blots)
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