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Mouse Aak1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q3UHJ0 |
| Other Accession | P0C1X8, F1SPM8, Q2M2I8, F1MH24, NP_808430.2, NP_001035195.1 |
| Reactivity | Mouse |
| Predicted | Bovine, Human, Pig, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 103346 Da |
| Antigen Region | 279-306 aa |
| Gene ID | 269774 |
|---|---|
| Other Names | AP2-associated protein kinase 1, Adaptor-associated kinase 1, Aak1, Kiaa1048 |
| Target/Specificity | This Mouse Aak1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 279-306 amino acids from the Central region of mouse Aak1. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Mouse Aak1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Aak1 |
|---|---|
| Synonyms | Kiaa1048 |
| Function | Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Preferentially, may phosphorylate substrates on threonine residues. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity. |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:F1MH24}; Peripheral membrane protein {ECO:0000250|UniProtKB:F1MH24}. Membrane, clathrin-coated pit {ECO:0000250|UniProtKB:P0C1X8}. Presynapse {ECO:0000250|UniProtKB:P0C1X8}. Note=Active when found in clathrin- coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells. {ECO:0000250|UniProtKB:P0C1X8} |
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Provided below are standard protocols that you may find useful for product applications.
Background
Aak1 phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis (By similarity).
References
Munton, R.P., et al. Mol. Cell Proteomics 6(2):283-293(2007)
Nishimura, M., et al. DNA Res. 11(5):315-323(2004)
Jha, A., et al. J. Biol. Chem. 279(3):2281-2290(2004)
Zambrowicz, B.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100(24):14109-14114(2003)
Hansen, J., et al. Proc. Natl. Acad. Sci. U.S.A. 100(17):9918-9922(2003)
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