|Application ||WB, E|
|Calculated MW||63510 Da|
|Antigen Region||54-84 aa|
|Other Names||CCR4-NOT transcription complex subunit 4, 632-, CCR4-associated factor 4, E3 ubiquitin-protein ligase CNOT4, Potential transcriptional repressor NOT4Hp, CNOT4, NOT4|
|Target/Specificity||This CNOT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-84 amino acids from the N-terminal region of human CNOT4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CNOT4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Has E3 ubiquitin ligase activity, promoting ubiquitination and degradation of target proteins (PubMed:11823428, PubMed:22159038, PubMed:26575292). Involved in activation of the JAK/STAT pathway (PubMed:11823428, PubMed:22159038). Catalyzes ubiquitination of methylated RBM15 (PubMed:26575292).|
|Cellular Location||Cytoplasm. Nucleus|
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Provided below are standard protocols that you may find useful for product applications.
CNOT4 has intrinsic E3 ubiquitin ligase activity. Binding of the CNOT4 RING finger to the ubiquitin-conjugating enzyme (E2) UbcH5B is highly selective. The CCR4-NOT complex functions as a global regulator of RNA polymerase II transcription.
Albert T.K., Nucleic Acids Res. 28:809-817(2000).
Albert T.K., EMBO J. 21:355-364(2002).
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