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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   CNOT4 Antibody   

CNOT4 Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CNOT4 Antibody AP1462d
    Anti-CNOT4 Antibody (N-Term) at 1:2000 dilution + Mouse cerebellum lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 64 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - CNOT4 Antibody AP1462d
    Western blot analysis of CNOT4 Antibody (Cat. #AP1462d) in MCF-7, K562, 293 cell line lysates (35ug/lane). CNOT4 (arrow) was detected using the purified Pab.
  • IHC-P - CNOT4 Antibody AP1462d
    Formalin-fixed and paraffin-embedded human testis tissue reacted with CNOT4 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - CNOT4 Antibody AP1462d
    CNOT4 Antibody (Cat. #AP1462d) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession O95628
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 63510 Da
Additional Information
Gene ID 4850
Other Names CCR4-NOT transcription complex subunit 4, 632-, CCR4-associated factor 4, E3 ubiquitin-protein ligase CNOT4, Potential transcriptional repressor NOT4Hp, CNOT4, NOT4
Target/Specificity This CNOT4 antibody is generated from rabbits immunized with human CNOT4 recombinant protein.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCNOT4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CNOT4
Synonyms NOT4
Function Has E3 ubiquitin ligase activity. Involved in activation of the JAK/STAT pathway.
Cellular Location Cytoplasm. Nucleus
Research Areas
Citations (0)

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Background

CNOT4 has intrinsic E3 ubiquitin ligase activity. Binding of the CNOT4 RING finger to the ubiquitin-conjugating enzyme (E2) UbcH5B is highly selective. The CCR4-NOT complex functions as a global regulator of RNA polymerase II transcription.

References

Albert T.K., Nucleic Acids Res. 28:809-817(2000).
Albert T.K., EMBO J. 21:355-364(2002).

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$ 295.00
$ 99.00
Cat# AP1462d
Size:
Quantity:
(40 western blots)
Availability: In Stock
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