|Application ||WB, E|
|Calculated MW||100307 Da|
|Antigen Region||651-679 aa|
|Other Names||DNA polymerase nu, POLN|
|Target/Specificity||This POLN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 651-679 amino acids from the C-terminal region of human POLN.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||POLN Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||DNA polymerase with very low fidelity that catalyzes considerable misincorporation by inserting dTTP opposite a G template, and dGTP opposite a T template (PubMed:16787914, PubMed:17118716). Is the least accurate of the DNA polymerase A family (i.e. POLG, POLN and POLQ) (PubMed:17118716). Can perform accurate translesion DNA synthesis (TLS) past a 5S-thymine glycol. Can perform efficient strand displacement past a nick or a gap and gives rise to an amount of product similar to that on non-damaged template. Has no exonuclease activity (PubMed:16787914). Error- prone DNA polymerase that preferentially misincorporates dT regardless of template sequence (PubMed:25775266). May play a role in TLS during interstrand cross-link (ICL) repair (PubMed:19908865). May be involved in TLS when genomic replication is blocked by extremely large major groove DNA lesions. May function in the bypass of some DNA-protein and DNA-DNA cross- links. May have a role in cellular tolerance to DNA cross-linking agents (PubMed:20102227). Involved in the repair of DNA cross- links and double-strand break (DSB) resistance. Participates in FANCD2-mediated repair. Forms a complex with HELQ helicase that participates in homologous recombination (HR) repair and is essential for cellular protection against DNA cross-links (PubMed:19995904).|
|Tissue Location||Highly expressed in testis and heart. Weakly expressed in skeletal muscle.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
The function of this protein remains unknown.
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Yamanaka, K., et al. Chem. Res. Toxicol. 23(3):689-695(2010)
Moldovan, G.L., et al. Mol. Cell. Biol. 30(4):1088-1096(2010)
Zietlow, L., et al. Biochemistry 48(49):11817-11824(2009)
Arana, M.E., et al. DNA Repair (Amst.) 6(2):213-223(2007)
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