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VAMP3 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - VAMP3 Antibody (Center) AP14660c
    VAMP3 Antibody (Center) (Cat. #AP14660c) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the VAMP3 antibody detected the VAMP3 protein (arrow).
  • WB - VAMP3 Antibody (Center) AP14660c
    VAMP3 Antibody (Center) (Cat. #AP14660c) western blot analysis in zebra fish brain tissue lysates (35ug/lane).This demonstrates the VAMP3 antibody detected the VAMP3 protein (arrow).
  • IHC-P - VAMP3 Antibody (Center) AP14660c
    VAMP3 Antibody (Center) (AP14660c)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VAMP3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q15836
Other Accession P63025, P63024, Q4R8T0, Q2KJD2, P63045, P63044, P63027, P63026, Q63666, Q62442, P23763, Q0V7N0, NP_004772.1
Reactivity Human, Mouse, Zebrafish
Predicted Bovine, Rat, Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 11309 Da
Antigen Region 15-43 aa
Additional Information
Gene ID 9341
Other Names Vesicle-associated membrane protein 3, VAMP-3, Cellubrevin, CEB, Synaptobrevin-3, VAMP3, SYB3
Target/Specificity This VAMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-43 amino acids from the Central region of human VAMP3.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVAMP3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VAMP3
Synonyms SYB3
Function SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.
Cellular Location Membrane; Single-pass type IV membrane protein. Cell junction, synapse, synaptosome
Research Areas
Citations (0)

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Background

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein encoded by this gene was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation.

References

Ban, H.J., et al. BMC Genet. 11, 26 (2010) :
Fader, C.M., et al. Biochim. Biophys. Acta 1793(12):1901-1916(2009)
Kean, M.J., et al. J. Cell. Sci. 122 (PT 22), 4089-4098 (2009) :
Vassilopoulos, S., et al. Science 324(5931):1192-1196(2009)
Luftman, K., et al. Biochem. Biophys. Res. Commun. 380(1):65-70(2009)

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Cat# AP14660c
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(40 western blots)
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