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IL25 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - IL25 Antibody (Center) AP14661c
    All lanes : Anti-IL25 Antibody (Center) at 1:1000 dilution Lane 1: human testis lysate Lane 2: PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - IL25 Antibody (Center) AP14661c
    IL25 Antibody (Center) (Cat. #AP14661c) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the IL25 antibody detected the IL25 protein (arrow).
  • IHC-P - IL25 Antibody (Center) AP14661c
    IL25 Antibody (Center) (AP14661c)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IL25 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H293
Other Accession NP_073626.1, NP_758525.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 20330 Da
Antigen Region 76-104 aa
Additional Information
Gene ID 64806
Other Names Interleukin-25, IL-25, Interleukin-17E, IL-17E, IL25, IL17E
Target/Specificity This IL25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-104 amino acids from the Central region of human IL25.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIL25 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IL25
Synonyms IL17E
Function Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.
Cellular Location Secreted.
Tissue Location Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea
Research Areas
Citations (0)

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The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants.


Xu, G., et al. Allergy 65(5):581-589(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Goswami, S., et al. Nat. Immunol. 10(5):496-503(2009)
Caruso, R., et al. Blood 113(15):3512-3519(2009)
Garley, M., et al. Adv Med Sci 53(2):326-330(2008)

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$ 295.00
$ 99.00
Cat# AP14661c
(40 western blots)
Availability: In Stock
Bulk Size
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