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HMGB3 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - HMGB3 Antibody (Center) AP14662c
    Anti-HMGB3 Antibody (Center) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - HMGB3 Antibody (Center) AP14662c
    HMGB3 Antibody (Center) (Cat. #AP14662c) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the HMGB3 antibody detected the HMGB3 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O15347
Other Accession O54879, P40618, Q32L31, NP_005333.2
Reactivity Human
Predicted Bovine, Chicken, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 22980 Da
Antigen Region 50-79 aa
Additional Information
Gene ID 3149
Other Names High mobility group protein B3, High mobility group protein 2a, HMG-2a, High mobility group protein 4, HMG-4, HMGB3, HMG2A, HMG4
Target/Specificity This HMGB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-79 amino acids from the Central region of human HMGB3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHMGB3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HMGB3
Synonyms HMG2A, HMG4
Function Binds preferentially single-stranded DNA and unwinds double-stranded DNA.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00267}. Chromosome
Tissue Location Expressed predominantly in placenta.
Citations (0)

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HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription (Wilke et al., 1997 [PubMed 9370291]; Nemeth et al., 2006 [PubMed 16945912]).


Nemeth, M.J., et al. Proc. Natl. Acad. Sci. U.S.A. 103(37):13783-13788(2006)
Ross, M.T., et al. Nature 434(7031):325-337(2005)
Vaccari, T., et al. Genomics 49(2):247-252(1998)
Wilke, K., et al. Gene 198 (1-2), 269-274 (1997) :
Davis, D.L., et al. Gene 113(2):251-256(1992)

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$ 295.00
$ 99.00
Cat# AP14662c
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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