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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PLD2 Antibody (N-term)   

PLD2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PLD2 Antibody (N-term) AP14669a
    Western blot analysis of lysate from human heart tissue lysate, using PLD2 Antibody (N-term)(Cat. #AP14669a). AP14669a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • WB - PLD2 Antibody (N-term) AP14669a
    PLD2 Antibody (N-term) (Cat. #AP14669a) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the PLD2 antibody detected the PLD2 protein (arrow).
  • WB - PLD2 Antibody (N-term) AP14669a
    PLD2 Antibody (N-term) (Cat. #AP14669a) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the PLD2 antibody detected the PLD2 protein (arrow).
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O14939
Other Accession Q0V8L6, NP_002654.3
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 105987 Da
Antigen Region 10-39 aa
Additional Information
Gene ID 5338
Other Names Phospholipase D2, PLD 2, hPLD2, Choline phosphatase 2, PLD1C, Phosphatidylcholine-hydrolyzing phospholipase D2, PLD2
Target/Specificity This PLD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-39 amino acids from the N-terminal region of human PLD2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPLD2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PLD2
Function May have a role in signal-induced cytoskeletal regulation and/or endocytosis.
Cellular Location Membrane; Peripheral membrane protein
Tissue Location Ubiquitous.
Research Areas
Citations ( 0 )

Background

Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.[supplied by OMIM].

References

Chae, Y.C., et al. Mol. Cell. Biol. 30(21):5086-5098(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Knapek, K., et al. Mol. Cell. Biol. 30(18):4492-4506(2010)
Tabatabaian, F., et al. J. Biol. Chem. 285(25):18991-19001(2010)
Kang, D.W., et al. PLoS ONE 5 (8), E12109 (2010) :

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Discontinued
Cat# AP14669a
Size:
(40 western blots)
Alternative Products:

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