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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   CD274 Antibody (C-term)   

CD274 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CD274 Antibody (C-term) AP14682b
    All lanes : Anti-CD274 Antibody (C-term) at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: HCT116 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - CD274 Antibody (C-term) AP14682b
    Anti-CD274 Antibody (C-term) at 1:2000 dilution + A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9NZQ7
Other Accession NP_054862.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 33275 Da
Antigen Region 261-290 aa
Additional Information
Gene ID 29126
Other Names Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274, CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1
Target/Specificity This CD274 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 261-290 amino acids from the C-terminal region of human CD274.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD274 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD274
Synonyms B7H1, PDCD1L1, PDCD1LG1, PDL1
Function Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
Cellular Location Isoform 1: Cell membrane; Single-pass type I membrane protein
Tissue Location Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
Research Areas
Citations (0)

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Background

Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

References

Berthon, C., et al. Cancer Immunol. Immunother. 59(12):1839-1849(2010)
Dianzani, C., et al. J. Immunol. 185(7):3970-3979(2010)
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Alvarez, I.B., et al. J. Infect. Dis. 202(4):524-532(2010)
Francisco, L.M., et al. Immunol. Rev. 236, 219-242 (2010) :

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$ 295.00
$ 99.00
Cat# AP14682b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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