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TBX5 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - TBX5 Antibody (N-term) AP14687A
    All lanes : Anti-TBX5 Antibody (N-term) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: HepG2 whole cell lysates Lane 3: HL-60 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - TBX5 Antibody (N-term) AP14687A
    TBX5 Antibody (N-term) (Cat. #AP14687a) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the TBX5 antibody detected the TBX5 protein (arrow).
  • IHC-P - TBX5 Antibody (N-term) AP14687A
    TBX5 Antibody (N-term) (AP14687a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TBX5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99593
Other Accession NP_542449.1, NP_852259.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 57711 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 6910
Other Names T-box transcription factor TBX5, T-box protein 5, TBX5
Target/Specificity This TBX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TBX5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTBX5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TBX5
Function Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00201}
Research Areas
Citations (0)

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This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene.


Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Stirnimann, C.U., et al. J. Mol. Biol. 400(1):71-81(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Hong, K.W., et al. J. Hum. Genet. 55(6):336-341(2010)

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$ 295.00
$ 99.00
Cat# AP14687A
(40 western blots)
Availability: In Stock
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