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Mouse Ephb4 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Mouse Ephb4 Antibody (Center) AP14714C
    Mouse Ephb4 Antibody (Center) (Cat. #AP14714c) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Ephb4 antibody detected the Ephb4 protein (arrow).
  • WB - Mouse Ephb4 Antibody (Center) AP14714C
    Western blot analysis of Ephb4 (arrow) using rabbit polyclonal Mouse Ephb4 Antibody (Center) (Cat. #AP14714c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Ephb4 gene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P54761
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 108848 Da
Antigen Region 360-389 aa
Additional Information
Gene ID 13846
Other Names Ephrin type-B receptor 4, Developmental kinase 2, mDK-2, Hepatoma transmembrane kinase, Tyrosine kinase MYK-1, Ephb4, Htk, Mdk2, Myk1
Target/Specificity This Mouse Ephb4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 360-389 amino acids from the Central region of mouse Ephb4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Ephb4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Ephb4
Synonyms Htk, Mdk2, Myk1
Function Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4- mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis.
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location Expressed in various organ systems, including lung, liver, kidney, intestine, muscle and heart
Research Areas
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Receptor for members of the ephrin-B family. Binds to ephrin-B2.

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Cat# AP14714C
(40 western blots)
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