|Application ||WB, E|
|Other Accession||Q8CFI7, NP_000929.1|
|Calculated MW||133897 Da|
|Antigen Region||797-826 aa|
|Other Names||DNA-directed RNA polymerase II subunit RPB2, DNA-directed RNA polymerase II 140 kDa polypeptide, DNA-directed RNA polymerase II subunit B, RNA polymerase II subunit 2, RNA polymerase II subunit B2, POLR2B|
|Target/Specificity||This POLR2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 797-826 amino acids from the Central region of human POLR2B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||POLR2B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. [provided by RefSeq].
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Michiels, S., et al. Carcinogenesis 30(5):763-768(2009)
Tu, S., et al. Nat. Struct. Mol. Biol. 15(4):419-421(2008)
Lee, J.H., et al. Mol. Cell. Biol. 28(2):609-618(2008)
Yamaguchi, Y., et al. Genes Cells 12(7):863-875(2007)
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