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POLR2B Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - POLR2B Antibody (Center) AP14739c
    POLR2B Antibody (Center) (Cat. #AP14739c) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the POLR2B antibody detected the POLR2B protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P30876
Other Accession Q8CFI7, NP_000929.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 133897 Da
Antigen Region 797-826 aa
Additional Information
Gene ID 5431
Other Names DNA-directed RNA polymerase II subunit RPB2, DNA-directed RNA polymerase II 140 kDa polypeptide, DNA-directed RNA polymerase II subunit B, RNA polymerase II subunit 2, RNA polymerase II subunit B2, POLR2B
Target/Specificity This POLR2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 797-826 amino acids from the Central region of human POLR2B.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOLR2B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).
Cellular Location Nucleus.
Citations (0)

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This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. [provided by RefSeq].


Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Michiels, S., et al. Carcinogenesis 30(5):763-768(2009)
Tu, S., et al. Nat. Struct. Mol. Biol. 15(4):419-421(2008)
Lee, J.H., et al. Mol. Cell. Biol. 28(2):609-618(2008)
Yamaguchi, Y., et al. Genes Cells 12(7):863-875(2007)

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$ 295.00
$ 99.00
Cat# AP14739c
(40 western blots)
Availability: In Stock
Bulk Size
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