|Application ||WB, IHC-P, E|
|Other Accession||Q2KIZ8, NP_005906.2|
|Calculated MW||92889 Da|
|Antigen Region||80-109 aa|
|Other Names||DNA replication licensing factor MCM6, p105MCM, MCM6|
|Target/Specificity||This MCM6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-109 amino acids from the N-terminal region of human MCM6.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MCM6 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.|
|Cellular Location||Nucleus. Note=Binds to chromatin during G1 and detach from it during S phase|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication.
Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
Timpson, N.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1341-1348(2010)
Wei, Z., et al. J. Biol. Chem. 285(17):12469-12473(2010)
Upton, J., et al. N. Z. Med. J. 123 (1308), 123 (2010) :
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :
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