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BRDG 1 Antibody (N-term F56)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - BRDG 1 Antibody (N-term F56) AP1475A
    Western blot analysis of anti-STAP1 Antibody (N-term) (F56) (Cat.#AP1475a) in mouse kidney tissue lysates (35ug/lane). STAP(arrow) was detected using the purified Pab.
  • WB - BRDG 1 Antibody (N-term F56) AP1475A
    Western blot analysis of STAP1 (arrow) using rabbit polyclonal STAP1 Antibody (N-term) (F56) (Cat.#AP1475a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the STAP1 gene.
  • IHC-P - BRDG 1 Antibody (N-term F56) AP1475A
    Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with STAP1 antibody (N-term)(F56) (Cat.#AP1475a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9ULZ2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 34291 Da
Antigen Region 41-70 aa
Additional Information
Gene ID 26228
Other Names Signal-transducing adaptor protein 1, STAP-1, BCR downstream-signaling protein 1, Docking protein BRDG1, Stem cell adaptor protein 1, STAP1, BRDG1
Target/Specificity This BRDG 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from the N-terminal region of human BRDG 1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBRDG 1 Antibody (N-term F56) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name STAP1
Synonyms BRDG1
Function In BCR signaling, appears to function as a docking protein acting downstream of TEC and participates in a positive feedback loop by increasing the activity of TEC.
Cellular Location Nucleus. Cytoplasm. Mitochondrion
Research Areas
Citations (0)

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STAP1 functions as a docking protein acting downstream of Tec tyrosine kinase in B cell antigen receptor signaling. The protein is directly phosphorylated by Tec in vitro where it participates in a postive feedback loop, increasing Tec activity.


Ma,J., Atherosclerosis 191 (1), 63-72 (2007)
Beausoleil,S.A., Nat. Biotechnol. 24 (10), 1285-1292 (2006)
Gstaiger,M., Science 302 (5648), 1208-1212 (2003)
Minoguchi,M., J. Biol. Chem. 278 (13), 11182-11189 (2003)

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Cat# AP1475A
(40 western blots)
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