|Application ||WB, E|
|Other Accession||Q8C4Y3, NP_056271.2|
|Calculated MW||65697 Da|
|Antigen Region||256-285 aa|
|Other Names||Negative elongation factor B, NELF-B, Cofactor of BRCA1, NELFB, COBRA1, KIAA1182|
|Target/Specificity||This COBRA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-285 amino acids from the Central region of human COBRA1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||COBRA1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin unfolding.|
|Tissue Location||Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas|
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Provided below are standard protocols that you may find useful for product applications.
NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).
Sun, J., et al. J. Cell. Biochem. 103(6):1798-1807(2008)
Sun, J., et al. J. Steroid Biochem. Mol. Biol. 107 (3-5), 131-139 (2007) :
Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Narita, T., et al. Mol. Cell 26(3):349-365(2007)
Aiyar, S.E., et al. Int. J. Biol. Sci. 3(7):486-492(2007)
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