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BRF1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - BRF1 Antibody (Center) AP14801C
    BRF1 Antibody (Center) (Cat. #AP14801c) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the BRF1 antibody detected the BRF1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q92994
Other Accession NP_663718.1, NP_001510.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 73840 Da
Antigen Region 314-342 aa
Additional Information
Gene ID 2972
Other Names Transcription factor IIIB 90 kDa subunit, TFIIIB90, hTFIIIB90, B-related factor 1, BRF-1, hBRF, TAF3B2, TATA box-binding protein-associated factor, RNA polymerase III, subunit 2, BRF1, BRF, GTF3B, TAF3B2, TAF3C
Target/Specificity This BRF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314-342 amino acids from the Central region of human BRF1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBRF1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BRF1
Synonyms BRF, GTF3B, TAF3B2, TAF3C
Function General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.
Cellular Location Nucleus.
Citations (0)

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This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Two alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. Other transcript variants are possible, but their full-length natures have not been completely characterized. [provided by RefSeq].


Haurie, V., et al. Proc. Natl. Acad. Sci. U.S.A. 107(9):4176-4181(2010)
Maitra, S., et al. RNA 14(5):950-959(2008)
Leal, J.F., et al. Oncogene 27(14):1961-1970(2008)
Yoo, N.K., et al. Cell. Microbiol. 10(3):583-592(2008)
Cabarcas, S., et al. BMC Mol. Biol. 9, 74 (2008) :

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$ 295.00
$ 99.00
Cat# AP14801C
(40 western blots)
Availability: In Stock
Bulk Size
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