PJA1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
---|---|
Primary Accession | Q8NG27 |
Other Accession | NP_071763.2, NP_660095.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 71002 Da |
Antigen Region | 155-183 aa |
Gene ID | 64219 |
---|---|
Other Names | E3 ubiquitin-protein ligase Praja-1, Praja1, 632-, RING finger protein 70, PJA1, RNF70 |
Target/Specificity | This PJA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-183 amino acids from the N-terminal region of human PJA1. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PJA1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PJA1 |
---|---|
Synonyms | RNF70 |
Function | Has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteasome (By similarity). May be involved in protein sorting. |
Tissue Location | Expressed in various regions of the brain including the cerebellum, cerebral cortex, medulla, occipital pole, frontal lobe, temporal lobe and putamen. Highest levels in the cerebral cortex |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes an enzyme that has E2-dependent E3 ubiquitin-protein ligase activity. This enzyme belongs to a class of ubiquitin ligases that include a RING finger motif, and it can interact with the E2 ubiquitin-conjugating enzyme UbcH5B. This gene is located in an area of chromosome X where several X-linked mental retardation disorders have been associated, and it has also been found as part of a contiguous gene deletion associated with craniofrontonasal syndrome, though a direct link to any disorder has yet to be demonstrated. Alternative splicing results in multiple transcript variants.
References
Wieland, I., et al. Clin. Genet. 72(6):506-516(2007)
Saha, T., et al. Oncogene 25(5):693-705(2006)
Mishra, L., et al. Cancer Biol. Ther. 4(7):694-699(2005)
Sasaki, A., et al. J. Biol. Chem. 277(25):22541-22546(2002)
Yu, P., et al. Genomics 79(6):869-874(2002)
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