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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   PJA1 Antibody (N-term)   

PJA1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PJA1 Antibody (N-term) AP14811A
    PJA1 Antibody (N-term) (Cat. #AP14811a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PJA1 antibody detected the PJA1 protein (arrow).
  • IHC-P - PJA1 Antibody (N-term) AP14811A
    PJA1 Antibody (N-term) (AP14811a)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PJA1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8NG27
Other Accession NP_071763.2, NP_660095.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 71002 Da
Antigen Region 155-183 aa
Additional Information
Gene ID 64219
Other Names E3 ubiquitin-protein ligase Praja-1, Praja1, 632-, RING finger protein 70, PJA1, RNF70
Target/Specificity This PJA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-183 amino acids from the N-terminal region of human PJA1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPJA1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PJA1
Synonyms RNF70
Function Has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteasome (By similarity). May be involved in protein sorting.
Tissue Location Expressed in various regions of the brain including the cerebellum, cerebral cortex, medulla, occipital pole, frontal lobe, temporal lobe and putamen. Highest levels in the cerebral cortex.
Research Areas
Citations (0)

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This gene encodes an enzyme that has E2-dependent E3 ubiquitin-protein ligase activity. This enzyme belongs to a class of ubiquitin ligases that include a RING finger motif, and it can interact with the E2 ubiquitin-conjugating enzyme UbcH5B. This gene is located in an area of chromosome X where several X-linked mental retardation disorders have been associated, and it has also been found as part of a contiguous gene deletion associated with craniofrontonasal syndrome, though a direct link to any disorder has yet to be demonstrated. Alternative splicing results in multiple transcript variants.


Wieland, I., et al. Clin. Genet. 72(6):506-516(2007)
Saha, T., et al. Oncogene 25(5):693-705(2006)
Mishra, L., et al. Cancer Biol. Ther. 4(7):694-699(2005)
Sasaki, A., et al. J. Biol. Chem. 277(25):22541-22546(2002)
Yu, P., et al. Genomics 79(6):869-874(2002)

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$ 295.00
$ 99.00
Cat# AP14811A
(40 western blots)
Availability: In Stock
Bulk Size
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