|Application ||WB, IHC-P, E|
|Other Accession||NP_071763.2, NP_660095.1|
|Calculated MW||71002 Da|
|Antigen Region||155-183 aa|
|Other Names||E3 ubiquitin-protein ligase Praja-1, Praja1, 632-, RING finger protein 70, PJA1, RNF70|
|Target/Specificity||This PJA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-183 amino acids from the N-terminal region of human PJA1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PJA1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteasome (By similarity). May be involved in protein sorting.|
|Tissue Location||Expressed in various regions of the brain including the cerebellum, cerebral cortex, medulla, occipital pole, frontal lobe, temporal lobe and putamen. Highest levels in the cerebral cortex.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes an enzyme that has E2-dependent E3 ubiquitin-protein ligase activity. This enzyme belongs to a class of ubiquitin ligases that include a RING finger motif, and it can interact with the E2 ubiquitin-conjugating enzyme UbcH5B. This gene is located in an area of chromosome X where several X-linked mental retardation disorders have been associated, and it has also been found as part of a contiguous gene deletion associated with craniofrontonasal syndrome, though a direct link to any disorder has yet to be demonstrated. Alternative splicing results in multiple transcript variants.
Wieland, I., et al. Clin. Genet. 72(6):506-516(2007)
Saha, T., et al. Oncogene 25(5):693-705(2006)
Mishra, L., et al. Cancer Biol. Ther. 4(7):694-699(2005)
Sasaki, A., et al. J. Biol. Chem. 277(25):22541-22546(2002)
Yu, P., et al. Genomics 79(6):869-874(2002)
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