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LALBA Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - LALBA Antibody (Center) AP14834c
    AP14834c staining LALBA in Human breast tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - LALBA Antibody (Center) AP14834c
    Anti-LALBA Antibody (Center)at 1:2000 dilution + human breast lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 16 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - LALBA Antibody (Center) AP14834c
    Anti-LALBA Antibody (Center)at 1:2000 dilution + human breast lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 16 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - LALBA Antibody (Center) AP14834c
    Anti-LALBA Antibody (Center)at 1:2000 dilution + human breast lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 16 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - LALBA Antibody (Center) AP14834c
    Anti-LALBA Antibody (Center)at 1:2000 dilution + human breast lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 16 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - LALBA Antibody (Center) AP14834c
    LALBA Antibody (Center) (Cat. #AP14834c) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the LALBA antibody detected the LALBA protein (arrow).
  • IHC-P - LALBA Antibody (Center) AP14834c
    LALBA Antibody (Center) (AP14834c)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LALBA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P00709
Other Accession NP_002280.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 16225 Da
Antigen Region 39-68 aa
Additional Information
Gene ID 3906
Other Names Alpha-lactalbumin, Lactose synthase B protein, Lysozyme-like protein 7, LALBA, LYZL7
Target/Specificity This LALBA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the Central region of human LALBA.
Dilution IHC-P~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLALBA Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LALBA
Synonyms LYZL7
Function Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins.
Cellular Location Secreted.
Tissue Location Mammary gland specific. Secreted in milk.
Research Areas
Citations (0)

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Background

This gene encodes alpha-lactalbumin, a principal protein of milk. Alpha-lactalbumin forms the regulatory subunit of the lactose synthase (LS) heterodimer and beta 1,4-galactosyltransferase (beta4Gal-T1) forms the catalytic component. Together, these proteins enable LS to produce lactose by transfering galactose moieties to glucose. As a monomer, alpha-lactalbumin strongly binds calcium and zinc ions and may possess bactericidal or antitumor activity. A folding variant of alpha-lactalbumin, called HAMLET, likely induces apoptosis in tumor and immature cells.

References

Tolin, S., et al. FEBS J. 277(1):163-173(2010)
Rosner, H.I., et al. J. Mol. Biol. 394(2):351-362(2009)
Wang, J., et al. J. Dairy Sci. 91(12):4466-4476(2008)
Ramboarina, S., et al. J. Am. Chem. Soc. 130(46):15318-15326(2008)
Morozova, O.B., et al. J Phys Chem B 109(12):5912-5918(2005)

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$ 295.00
$ 99.00
Cat# AP14834c
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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