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SCF (KITLG) Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Alternative | |||
| AP1484a | AP1484b | DISCONTINED INQUIRE CLICK INQUIRE Add to cart | ||
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
SCF (KITLG) Antibody (N-term) - Product info | |
| Application | WB, FC
|
| Primary Accession | P21583 |
| Other Accession | Q28132 |
| Reactivity | Human |
| Predicted | Bovine |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 30899 Da |
SCF (KITLG) Antibody (N-term) - Additional info | |
| Gene ID 4254 | |
| Other Names KITLG; MGF; SCF; Kit ligand; Mast cell growth factor; Stem cell factor; c-Kit ligand; Soluble KIT ligand | |
| Target/Specificity This SCF (KITLG) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30~60 amino acids from the N-terminal region of human KITLG. | |
| Dilution WB~~1:100~500WB~~1:1000 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions SCF (KITLG) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
SCF (KITLG) Antibody (N-term) - Protein Information | |
| Name KITLG | |
| Synonyms MGF, SCF | |
| Function Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins | |
| Cellular Location Isoform 1: Cell membrane; Single-pass type I membrane protein Soluble KIT ligand: Secreted. | |
SCF (KITLG) Antibody (N-term) - Related products
AP1484a: SCF (KITLG) Antibody (N-term)
AP1484b: SCF (KITLG) Antibody (C-term)
AP1484c: SCF (KITLG) Antibody (Center)
RI12903: KITLG predesign siRNA
DC08836: Human KITLG cDNA Clone
LY11916a: KITLG Over-expression Lysate
BP1484a: KITLG Antibody (N-term) Blocking Peptide
BP1484b: KITLG Antibody (C-term) Blocking Peptide
SCF (KITLG) Antibody (N-term) - Application data
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Western blot analysis of KITLG (arrow) using rabbit polyclonal KITLG Antibody (N-term) (Cat.#AP1484a).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KITLG gene (Lane 2) (Origene Technologies).
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Flow cytometric analysis of WiDr cells using SCF (KITLG) Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
SCF (KITLG) Antibody (N-term) - Research Areas
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BACKGROUND
KITLG is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis.
REFERENCES
Young,S.M., Cell. Signal. 19 (12), 2572-2581 (2007) Pick,M., Stem Cells 25 (9), 2206-2214 (2007) Yasuda,A., Dig. Dis. Sci. 52 (9), 2292-2300 (2007)
