|Application ||WB, E|
|Other Accession||A6NIZ1, Q7ZXH7, Q62636, Q99JI6, Q4R9D4, Q5ZHX1, P61223, NP_056461.1, NP_001010942.1|
|Predicted||Bovine, Chicken, Monkey, Mouse, Rat, Xenopus|
|Calculated MW||20825 Da|
|Antigen Region||150-178 aa|
|Other Names||Ras-related protein Rap-1b, GTP-binding protein smg p21B, RAP1B|
|Target/Specificity||This RAP1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-178 amino acids from the C-terminal region of human RAP1B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RAP1B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function.|
|Cellular Location||Cell membrane. Cytoplasm, cytosol. Cell junction. Note=May shuttle between plasma membrane and cytosol Presence of KRIT1 and CDH5 is required for its localization to the cell junction|
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Provided below are standard protocols that you may find useful for product applications.
RAP1B and RAP1A (MIM 179520) belong to a superfamily of RAS (see MIM 190020)-like small GTP-binding proteins involved in cell signaling.
Kamatani, Y., et al. Nat. Genet. 42(3):210-215(2010)
Malchinkhuu, E., et al. Mol. Biol. Cell 20(24):5156-5165(2009)
Edreira, M.M., et al. J. Biol. Chem. 284(40):27480-27486(2009)
Matsuse, M., et al. Endocr. J. 56(1):161-164(2009)
Li, Y.H., et al. J. Biol. Chem. 283(48):33784-33792(2008)
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