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LIN28B Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP1485b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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LIN28B Antibody (C-term) - Product info | |
| Application | WB, FC
|
| Primary Accession | Q6ZN17 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 27084 Da |
LIN28B Antibody (C-term) - Additional info | |
| Gene ID 389421 | |
| Other Names LIN28B; CSDD2; Protein lin-28 homolog B | |
| Target/Specificity This LIN28B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221~250 amino acids from the C-terminal region of human LIN28B. | |
| Dilution WB~~1:50~100WB~~1:1000 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions LIN28B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
LIN28B Antibody (C-term) - Protein Information | |
| Name LIN28B | |
| Synonyms CSDD2 | |
| Function Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre- let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth | |
| Cellular Location Cytoplasm. Nucleus. Note=Predominantly cytoplasmic at G1 phase, accumulates in the nucleus in S and G2 phases. The frequency of nuclear localization in S and in G2 phases is 60% and 30%, respectively | |
| Tissue Location High expression in testis, fetal liver, placenta and in hepatocellular carcinoma (HCC). Isoform 1 is only detected in moderately and poorly differentiated HCC tissues and placenta (at protein level). Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level) | |
LIN28B Antibody (C-term) - Related products
AP1485a: LIN28B Antibody (N-term)
AP1485b: LIN28B Antibody (C-term)
AP1485c: LIN28B Antibody (Center)
RI13035: LIN28B predesign siRNA
BP1485a: LIN28B Antibody (N-term) Blocking Peptide
LIN28B Antibody (C-term) - Application data
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Western blot analysis of LIN28B Antibody (C-term) in HL60 cell line lysates (35ug/lane). LIN28B (arrow) was detected using the purified Pab (1:60 dilution).
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Flow cytometric analysis of HL-60 cells using LIN28B Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
LIN28B Antibody (C-term) - Research Areas
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BACKGROUND
Lin-28 homolog B (LIN28B) is overexpressed in hepatocellular carcinoma. The heterochronic gene lin-28 is a key regulator of developmental timing in the nematode Caenorhabditis elegans. Similar with lin-28 proteins, LIN28B conserves a cold shock domain and a pair of CCHC zinc finger domains. Phylogenetic analysis suggests that they might arise as a result of duplication from an ancestral gene. Overexpression of LIN28B was noted in most HCC cell lines and clinical samples. A short LIN28B isoform was also identified in non-tumor liver tissue and fetal liver. Although predominantly localized in the cytoplasm, LIN28B protein shows cell cycle-dependent nuclear translocation in Huh7 cells. Induced expression of exogenous LIN28B in a tet-off cell line promoted cancer cell proliferation.
REFERENCES
Guo,Y., Gene 384, 51-61 (2006)
