|Application ||WB, IHC-P, E|
|Other Accession||Q6PFX9, NP_003738.2|
|Calculated MW||142039 Da|
|Antigen Region||55-82 aa|
|Other Names||Tankyrase-1, TANK1, ADP-ribosyltransferase diphtheria toxin-like 5, ARTD5, Poly [ADP-ribose] polymerase 5A, TNKS-1, TRF1-interacting ankyrin-related ADP-ribose polymerase, Tankyrase I, TNKS, PARP5A, PARPL, TIN1, TINF1, TNKS1|
|Target/Specificity||This TNKS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-82 amino acids from the N-terminal region of human TNKS.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TNKS Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||PARP5A, PARPL, TIN1, TINF1, TNKS1|
|Function||Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length. Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI. May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. May be involved in spindle pole assembly through PARsylation of NUMA1. Stimulates 26S proteasome activity.|
|Cellular Location||Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Chromosome, centromere. Nucleus, nuclear pore complex. Chromosome, telomere Cytoplasm, cytoskeleton, spindle pole. Note=Associated with the Golgi and with juxtanuclear SLC2A4/GLUT4-vesicles. A minor proportion is also found at nuclear pore complexes and around the pericentriolar matrix of mitotic centromeres. During interphase, a small fraction of TNKS is found in the nucleus, associated with TERF1. Localizes to spindle poles at mitosis onset via interaction with NUMA1|
|Tissue Location||Ubiquitous; highest levels in testis.|
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Provided below are standard protocols that you may find useful for product applications.
TNKS may regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. Has PARP activity and can modify TERF1, and thereby contribute to the regulation of telomere length.
Hatsugai, K., et al. FEBS Lett. 584(18):3885-3890(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Scherag, A., et al. PLoS Genet. 6 (4), E1000916 (2010) :
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