|Application ||WB, E|
|Other Accession||NP_945328.1, NP_945353.1, NP_004697.2|
|Calculated MW||102435 Da|
|Antigen Region||164-192 aa|
|Other Names||Rho guanine nucleotide exchange factor 1, 115 kDa guanine nucleotide exchange factor, p115-RhoGEF, p115RhoGEF, Sub15, ARHGEF1|
|Target/Specificity||This ARHGEF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-192 amino acids from the N-terminal region of human ARHGEF1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ARHGEF1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2- induced RhoA activation.|
|Cellular Location||Cytoplasm. Membrane. Note=Translocated to the membrane by activated GNA13 or LPA stimulation|
|Tissue Location||Ubiquitously expressed.|
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Provided below are standard protocols that you may find useful for product applications.
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Loirand, G., et al. Med Sci (Paris) 26 (6-7), 561-563 (2010) :
Takefuji, M., et al. J. Hum. Genet. 55(1):42-49(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Slattum, G., et al. J. Cell Biol. 186(5):693-702(2009)
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