|Application ||WB, FC, E|
|Calculated MW||36086 Da|
|Antigen Region||43-71 aa|
|Other Names||Necdin, NDN|
|Target/Specificity||This NDN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-71 amino acids from the N-terminal region of human NDN.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NDN Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle- promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences (By similarity).|
|Cellular Location||Perikaryon. Nucleus. Note=Neural perikarya, translocates to the nucleus of postmitotic neurons and interacts with the nuclear matrix|
|Tissue Location||Almost ubiquitous. Detected in fetal brain, lung, liver and kidney; in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Not detected in peripheral blood leukocytes. In brain, restricted to post- mitotic neurons|
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Provided below are standard protocols that you may find useful for product applications.
This intronless gene is located in the Prader-Willi syndrome deletion region. It is an imprinted gene and is expressed exclusively from the paternal allele. Studies in mouse suggest that the protein encoded by this gene may suppress growth in postmitotic neurons.
Chapman, E.J., et al. Mol. Carcinog. 48(11):975-981(2009)
Yoshikawa, K. No To Hattatsu 41(3):214-218(2009)
Liu, X., et al. Mol. Cell. Neurosci. 41(1):51-61(2009)
de Krom, M., et al. Biol. Psychiatry 65(7):625-630(2009)
Lau, J.C., et al. Nucleic Acids Res. 32(11):3376-3382(2004)
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