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>   home   >   Products   >   Primary Antibodies   >   TRIM27 Antibody (C-term)   

TRIM27 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - TRIM27 Antibody (C-term) AP14892B
    TRIM27 Antibody (C-term) (Cat. #AP14892b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TRIM27 antibody detected the TRIM27 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P14373
Other Accession Q62158, NP_006501.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 58490 Da
Antigen Region 453-482 aa
Additional Information
Gene ID 5987
Other Names Zinc finger protein RFP, 632-, RING finger protein 76, Ret finger protein, Tripartite motif-containing protein 27, TRIM27, RFP, RNF76
Target/Specificity This TRIM27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 453-482 amino acids from the C-terminal region of human TRIM27.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTRIM27 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TRIM27
Synonyms RFP, RNF76
Function E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T- cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASH1, leading to promote endosomal F-actin assembly (PubMed:23452853). Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double- stranded DNA.
Cellular Location Nucleus. Cytoplasm. Nucleus, PML body. Early endosome. Note=Nuclear or cytoplasmic depending on the cell type (By similarity). Colocalized with PML and EIF3S6 in nuclear bodies. Recruited to retromer-containing endosomes via interaction with MAGEL2 (PubMed:23452853). {ECO:0000250, ECO:0000269|PubMed:23452853}
Tissue Location Expressed in testis namely within the seminiferous tubules.
Citations (0)

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This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein.


Johnson, A.D., et al. Nat. Genet. 42(7):608-613(2010)
Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009) :
Tezel, G.G., et al. Pathol. Res. Pract. 205(6):403-408(2009)
Li, X., et al. Virology 360(2):419-433(2007)
Lim, J., et al. Cell 125(4):801-814(2006)

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$ 295.00
$ 99.00
Cat# AP14892B
(40 western blots)
Availability: In Stock
Bulk Size
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