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GLUT2 (SLC2A2) Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GLUT2 (SLC2A2) Antibody (N-term) AP1489a
    Western blot analysis of SLC2A2 Antibody (N-term) (Cat.#AP1489a) in HepG2 cell line lysates (35ug/lane). SLC2A2 (arrow) was detected using the purified Pab.
    detail
  • WB - GLUT2 (SLC2A2) Antibody (N-term) AP1489a
    Anti-SLC2A2 Antibody (N-term) at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • FC - GLUT2 (SLC2A2) Antibody (N-term) AP1489a
    Flow cytometric analysis of HL-60 cells using GLUT2 (SLC2A2) Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession P11168
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 57490 Da
Antigen Region 31-60 aa
Additional Information
Gene ID 6514
Other Names Solute carrier family 2, facilitated glucose transporter member 2, Glucose transporter type 2, liver, GLUT-2, SLC2A2, GLUT2
Target/Specificity This GLUT2 (SLC2A2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-60 amino acids from the N-terminal region of human GLUT2 (SLC2A2).
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGLUT2 (SLC2A2) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SLC2A2 (HGNC:11006)
Function Facilitative hexose transporter that mediates the transport of glucose, fructose and galactose (PubMed:8027028, PubMed:16186102, PubMed:23396969, PubMed:28083649, PubMed:8457197). Likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell (PubMed:8027028). May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney (PubMed:3399500). Also able to mediate the transport of dehydroascorbate (PubMed:23396969).
Cellular Location Cell membrane; Multi-pass membrane protein
Tissue Location Liver, insulin-producing beta cell, small intestine and kidney.
Research Areas
Citations ( 0 )

Background

Glucose transporter 2 isoform is an integral plasma membrane glycoprotein of the liver, islet beta cells, intestine, and kidney epithelium. It mediates facilitated bidirectional glucose transport. Because of its low affinity for glucose, it has been suggested as a glucose sensor.

References

Freitas,H.S., Nephron Physiol 105 (3), P42-P51 (2007) Laukkanen,O., Diabetes 54 (7), 2256-2260 (2005) Roncero,I., J. Neurochem. 88 (5), 1203-1210 (2004)

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$ 365.00
$ 140.00
Cat# AP1489a
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