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SMAD9 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - SMAD9 Antibody (Center) AP14903C
    Anti-SMAD9 Antibody (Center) at 1:1000 dilution + A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - SMAD9 Antibody (Center) AP14903C
    SMAD9 Antibody (Center) (Cat. #AP14903c) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SMAD9 antibody detected the SMAD9 protein (arrow).
  • IF - SMAD9 Antibody (Center) AP14903C
    Fluorescent confocal image of MCF-7 cell stained with SMAD9 Antibody (Center)(Cat#AP14903c). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD9 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).SMAD9 immunoreactivity is localized to cytoplasm and nucleus significantly.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O15198
Other Accession NP_005896.1, NP_001120689.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 52493 Da
Antigen Region 200-228 aa
Additional Information
Gene ID 4093
Other Names Mothers against decapentaplegic homolog 9, MAD homolog 9, Mothers against DPP homolog 9, Madh6, SMAD family member 9, SMAD 9, Smad9, SMAD9, MADH6, MADH9
Target/Specificity This SMAD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-228 amino acids from the Central region of human SMAD9.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSMAD9 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SMAD9
Synonyms MADH6, MADH9
Function Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).
Cellular Location Cytoplasm. Nucleus. Note=In the cytoplasm in the absence of ligand Migration to the nucleus when complexed with SMAD4 (By similarity).
Tissue Location Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney
Research Areas
Citations (0)

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The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.


Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Liu, Y., et al. FASEB J. 23(7):2299-2306(2009)
Su, D., et al. J. Biol. Chem. 284(18):12153-12164(2009)
Shintani, M., et al. J. Med. Genet. 46(5):331-337(2009)
Hoover, L.L., et al. Sci Signal 1 (46), PE48 (2008) :

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$ 295.00
$ 99.00
Cat# AP14903C
(40 western blots)
Availability: In Stock
Bulk Size
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