|Application ||WB, IHC-P, E|
|Other Accession||Q7TPB1, NP_006421.2|
|Calculated MW||57924 Da|
|Antigen Region||4-32 aa|
|Other Names||T-complex protein 1 subunit delta, TCP-1-delta, CCT-delta, Stimulator of TAR RNA-binding, CCT4, CCTD, SRB|
|Target/Specificity||This CCT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human CCT4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CCT4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.|
|Cellular Location||Cytoplasm. Melanosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
The chaperonin containing TCP1 (MIM 186980) complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin (see MIM 102560) and tubulin (see MIM 191130), as well as alpha-transducin (MIM 139330) (Won et al., 1998 [PubMed 9819444]).
Mukherjee, K., et al. BMC Evol. Biol. 10, 64 (2010) :
Zebol, J.R., et al. Int. J. Biochem. Cell Biol. 41(4):822-827(2009)
Chi, A., et al. J. Proteome Res. 5(11):3135-3144(2006)
Hillier, L.W., et al. Nature 434(7034):724-731(2005)
Imai, Y., et al. J. Biol. Chem. 278(51):51901-51910(2003)
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