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ABCG2 (BCRP) Antibody (Center)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1490c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
ABCG2 (BCRP) Antibody (Center) - Product info | |
| Application | WB, IF, FC
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| Primary Accession | Q9UNQ0 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 72314 Da |
ABCG2 (BCRP) Antibody (Center) - Additional info | |
| Gene ID 9429 | |
| Other Names ABCG2; MXR; ABCP; BCRP; BCRP1; ATP-binding cassette sub-family G member 2; Breast cancer resistance protein; CDw338; Mitoxantrone resistance-associated protein; Placenta-specific ATP-binding cassette transporter; CD_antigen=CD338 | |
| Target/Specificity This ABCG2 (BCRP) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 313~342 amino acids from the center region of human ABCG2. | |
| Dilution WB~~1:100~500WB~~1:1000 IF~~1:10~50 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ABCG2 (BCRP) Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ABCG2 (BCRP) Antibody (Center) - Protein Information | |
| Name ABCG2 | |
| Synonyms ABCP, BCRP, BCRP1, MXR | |
| Function Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP- dependent increase in the efflux of rhodamine 123 | |
| Cellular Location Cell membrane; Multi-pass membrane protein. | |
| Tissue Location Highly expressed in placenta. Low expression in small intestine, liver and colon | |
ABCG2 (BCRP) Antibody (Center) - Related products
AP1490b: ABCG2 (BCRP) Antibody (C-term)
AP1490c: ABCG2 (BCRP) Antibody (Center)
RI10030: ABCG2 predesign siRNA
ABCG2 (BCRP) Antibody (Center) - Application data
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Western blot analysis of ABCG2 Antibody (Center) in 293 cell line lysates (35ug/lane). ABCG2(arrow) was detected using the purified Pab (1:60 dilution).
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Confocal immunofluorescent analysis of ABCG2 (BCRP) Antibody (Center)(Cat#AP1490c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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ABCG2 (BCRP) Antibody (Center) (Cat. #AP1490c) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ABCG2 (BCRP) Antibody (Center) - Research Areas
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BACKGROUND
ABCG2 is a membrane-associated protein included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue.
REFERENCES
Xie,Y., J. Biol. Chem. 283 (6), 3349-3356 (2008) Tamura,A., Drug Metab. Pharmacokinet. 22 (6), 428-440 (2007)
