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Actin (ACTB/ACTC) Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)

Country
United States
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Ordering Information
Catalog # Size Availability Price  
AP1491a 0.1 mg 400 ul In Stock $ 255.00 Add to cart
  • Specification
  • Citiations : 0
  • Reviews
  • Protocols
  • Backgrounds

Actin (ACTB/ACTC) Antibody (N-term) - Product info

ApplicationWB, IHC, IF
  • Applications Legend:
  • W=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • F=Flow Cytometry
Primary AccessionP68032
Other AccessionP60010, P68136, P68135, P68137, P68134, P68133, P68139, P68138, P63269, P63268, P63267, P63270, Q5E9B5, A2BDB0, P63259, P63260, P63261, Q5ZMQ2, P63258, P04751, P68035, P68033, P68034, Q3ZC07, O93400, P60711, P29751, Q6QAQ1, P60710, Q4R561, P60709, P48975;
ReactivityHuman, Mouse
PredictedBovine, Chicken, Drosophila, C.Elegans, Hamster, Pig, Monkey, Rat, Rabbit, Xenopus, Yeast, Zebrafish
Concentration0.25 mg/ml
IsotypeRabbit Ig
Calculated MW42019 Da

Actin (ACTB/ACTC) Antibody (N-term) - Additional info

Gene ID 70
Other Names
ACTC1; ACTC; Actin, alpha cardiac muscle 1; Alpha-cardiac actin
Target/Specificity
This ACTB/ACTC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43~73 amino acids from the N-terminal region of human ACTB/ACTC.
Dilution
WB~~1:50~100
IHC~~1:10~50
IF~~1:10~50
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
Actin (ACTB/ACTC) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Actin (ACTB/ACTC) Antibody (N-term) - Protein Information

Name ACTC1
Synonyms ACTC
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells
Cellular Location
Cytoplasm, cytoskeleton.

Actin (ACTB/ACTC) Antibody (N-term) - Related products

AP1491a: Actin (ACTB/ACTC) Antibody (N-term)

AP1491c: Actin (ACTB/ACTC) Antibody (Center)

RI10087: ACTC1 predesign siRNA

DC02826: Human ACTC1 cDNA Clone

LY10836a: ACTC1 Over-expression Lysate

BP1491a: ACTB/ACTC Antibody (N-term) Blocking Peptide

BP1491c: ACTB/ACTC Antibody (Center) Blocking Peptide

AJ1012a: Actin Antibody

Actin (ACTB/ACTC) Antibody (N-term) - Application data

  • Western blot analysis of ACTB/ACTC Antibody (N-term) in mouse testis tissue lysates (35ug/lane). Target protein (arrow) was detected using the purified Pab (1:60 dilution).

  • Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ACTB/ACTC antibody (N-term) (Cat.#AP1491a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • Confocal immunofluorescent analysis of Actin (ACTB/ACTC) Antibody (N-term)(Cat#AP1491a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Actin (ACTB/ACTC) Antibody (N-term) - Research Areas

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. ACTB/ACTC are nonmuscle cytoskeletal actins and major constituents of the contractile apparatus. Defects in ACTB are a cause of juvenile-onset dystonia. Defects in ACTC have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).

REFERENCES

Villebeck,L., Biochemistry 46 (44), 12639-12647 (2007) Avizienyte,E., Exp. Cell Res. 313 (15), 3175-3188 (2007) Bouldin,A.A., Muscle Nerve 35 (2), 254-258 (2007)