POLG Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IF, FC, WB, IHC-P, E |
---|---|
Primary Accession | P54098 |
Other Accession | Q9QYV8, P54099, NP_001119603.1, NP_002684.1 |
Reactivity | Human, Mouse |
Predicted | Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 139562 Da |
Antigen Region | 1120-1148 aa |
Gene ID | 5428 |
---|---|
Other Names | DNA polymerase subunit gamma-1, Mitochondrial DNA polymerase catalytic subunit, PolG-alpha, POLG, MDP1, POLG1, POLGA |
Target/Specificity | This POLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1120-1148 amino acids from the C-terminal region of human POLG. |
Dilution | IF~~1:10~50 WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | POLG Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | POLG |
---|---|
Synonyms | MDP1, POLG1, POLGA |
Function | Involved in the replication of mitochondrial DNA. Associates with mitochondrial DNA. |
Cellular Location | Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid |
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Provided below are standard protocols that you may find useful for product applications.
Background
Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene.
References
Tong, Z.B., et al. Fertil. Steril. 94(7):2932-2934(2010)
Stewart, J.D., et al. Hepatology 52(5):1791-1796(2010)
Batabyal, D., et al. J. Biol. Chem. 285(44):34191-34201(2010)
Wang, W., et al. Nucleic Acids Res. (2010) In press :
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)
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