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NEDD4L Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NEDD4L Antibody (Center) AP14949C
    NEDD4L Antibody (Center) (Cat. #AP14949c) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the NEDD4L antibody detected the NEDD4L protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q96PU5
Other Accession Q8CFI0, NP_001138436.1, NP_001138442.1, NP_001138439.1
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 111932 Da
Antigen Region 576-604 aa
Additional Information
Gene ID 23327
Other Names E3 ubiquitin-protein ligase NEDD4-like, 632-, NEDD42, Nedd4-2, NEDD4L, KIAA0439, NEDL3
Target/Specificity This NEDD4L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 576-604 amino acids from the Central region of human NEDD4L.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNEDD4L Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NEDD4L
Synonyms KIAA0439, NEDL3
Function E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed:25631046). Plays a role in dendrite formation by melanocytes (PubMed:23999003).
Cellular Location Cytoplasm. Note=May be recruited to exosomes by NDFIP1
Tissue Location Ubiquitously expressed, with highest levels in prostate, pancreas and kidney (PubMed:14615060, PubMed:15496141, PubMed:19664597). Expressed in melanocytes (PubMed:23999003)
Research Areas
Citations (0)

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Background

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK.

References

Zhou, R., et al. J. Biol. Chem. 285(40):30523-30530(2010)
Raikwar, N.S., et al. Am. J. Physiol. Renal Physiol. 299 (2), F436-F444 (2010) :
Hallows, K.R., et al. J. Biol. Chem. 285(28):21671-21678(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Jin, H.S., et al. Kidney Blood Press. Res. 33(1):15-23(2010)

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$ 295.00
$ 99.00
Cat# AP14949C
Size:
Quantity:
(40 western blots)
Availability: In Stock
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