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SMURF1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SMURF1 Antibody (Center) AP14957c
    SMURF1 Antibody (Center) (Cat. #AP14957c) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the SMURF1 antibody detected the SMURF1 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9HCE7
Other Accession Q9CUN6, NP_851994.1, NP_065162.1
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 86114 Da
Antigen Region 352-381 aa
Additional Information
Gene ID 57154
Other Names E3 ubiquitin-protein ligase SMURF1, hSMURF1, 632-, SMAD ubiquitination regulatory factor 1, SMAD-specific E3 ubiquitin-protein ligase 1, SMURF1, KIAA1625
Target/Specificity This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-381 amino acids from the Central region of human SMURF1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSMURF1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SMURF1
Synonyms KIAA1625
Function E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Plays a role in dendrite formation by melanocytes (PubMed:23999003).
Cellular Location Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side
Tissue Location Expressed in melanocytes (PubMed:23999003).
Research Areas
Citations (0)

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Background

This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. A similar protein in Xenopus is involved in embryonic pattern formation. Alternative splicing results in multiple transcript variants encoding different isoforms. An additional transcript variant has been identified, but its full length sequence has not been determined.

References

Li, S., et al. Mol. Cell. Biochem. 338 (1-2), 11-17 (2010) :
Franke, A., et al. Nat. Genet. 42(4):292-294(2010)
Alarcon, C., et al. Cell 139(4):757-769(2009)
Crose, L.E., et al. J. Biol. Chem. 284(20):13301-13305(2009)
Narimatsu, M., et al. Cell 137(2):295-307(2009)

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Cat# AP14957c
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(40 western blots)
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