|Application ||WB, E|
|Other Accession||P12369, P31324, P31322, NP_002727.2|
|Predicted||Bovine, Mouse, Rat|
|Calculated MW||46302 Da|
|Antigen Region||119-147 aa|
|Other Names||cAMP-dependent protein kinase type II-beta regulatory subunit, PRKAR2B|
|Target/Specificity||This PRKAR2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-147 amino acids from the Central region of human PRKAR2B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PRKAR2B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.|
|Cellular Location||Cytoplasm. Cell membrane. Note=Colocalizes with PJA2 in the cytoplasm and at the cell membrane|
|Tissue Location||Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible|
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Provided below are standard protocols that you may find useful for product applications.
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol. [provided by RefSeq].
Liu, Y.J., et al. Obesity (Silver Spring) (2010) In press :
Adkins, D.E., et al. Mol. Psychiatry (2010) In press :
Islam, A., et al. J. Biol. Chem. 283(37):25364-25371(2008)
Ji, Z., et al. J. Biol. Chem. 283(32):21920-21925(2008)
Vincent-Dejean, C., et al. Eur. J. Endocrinol. 158(6):829-839(2008)
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