- CITATIONS: 1
|Application ||WB, IHC-P, FC, E|
|Calculated MW||51111 Da|
|Antigen Region||408-436 aa|
|Other Names||T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4, CD4|
|Target/Specificity||This CD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 408-436 amino acids from the C-terminal region of human CD4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CD4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum|
Provided below are standard protocols that you may find useful for product applications.
CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes (helper/inducer T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
Garron,M.L., J. Mol. Biol. 375 (5), 1320-1328 (2008) Rychert,J., J. Acquir. Immune Defic. Syndr. 46 (3), 261-267 (2007)
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