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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CD4 Antibody (C-term)   

CD4 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CD4 Antibody (C-term) AP1496b
    Western blot analysis of anti-CD4 Antibody (C-term) (Cat.#AP1496b) in mouse heart tissue lysates (35ug/lane).CD4 (arrow) was detected using the purified Pab.
  • IHC-P - CD4 Antibody (C-term) AP1496b
    Formalin-fixed and paraffin-embedded human Tonsil tissue reacted with CD4 antibody (C-term) (Cat.#AP1496b), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - CD4 Antibody (C-term) AP1496b
    Flow cytometric analysis of CEM cells using CD4 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IHC-P - CD4 Antibody (C-term) AP1496b
    Formalin-fixed and paraffin-embedded human Tonsil tissue reacted with CD4 antibody (C-term) (Cat.#AP1496b), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P01730
Other Accession P79185
Reactivity Human, Mouse
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 51111 Da
Antigen Region 408-436 aa
Additional Information
Gene ID 920
Other Names T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4, CD4
Target/Specificity This CD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 408-436 amino acids from the C-terminal region of human CD4.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD4
Function Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Cellular Location Cell membrane; Single-pass type I membrane protein Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum
Research Areas
Citations ( 0 )

Background

CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes (helper/inducer T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.

References

Garron,M.L., J. Mol. Biol. 375 (5), 1320-1328 (2008) Rychert,J., J. Acquir. Immune Defic. Syndr. 46 (3), 261-267 (2007)

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Discontinued
Cat# AP1496b
Size:
(40 western blots)
Alternative Products:

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