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CD4 Antibody (C-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1496b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
CD4 Antibody (C-term) - Product info | |
| Application | WB, IHC, FC
|
| Primary Accession | P01730 |
| Other Accession | P79185 |
| Reactivity | Human, Mouse |
| Predicted | Monkey |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 51111 Da |
CD4 Antibody (C-term) - Additional info | |
| Gene ID 920 | |
| Other Names CD4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD_antigen=CD4; Flags: Precursor | |
| Target/Specificity This CD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336~366 amino acids from the C-terminal region of human CD4. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions CD4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
CD4 Antibody (C-term) - Protein Information | |
| Name CD4 | |
| Function Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts | |
| Cellular Location Cell membrane; Single-pass type I membrane protein. Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum | |
CD4 Antibody (C-term) - Related products
AP1496a: CD4 Antibody (N-term)
AP1496b: CD4 Antibody (C-term)
LY11517a: CD4 Over-expression Lysate
BP1496a: CD4 Antibody (N-term) Blocking Peptide
CD4 Antibody (C-term) - Application data
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Western blot analysis of anti-CD4 Antibody (C-term) (Cat.#AP1496b) in mouse heart tissue lysates (35ug/lane).CD4 (arrow) was detected using the purified Pab (1:60 dilution).
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Formalin-fixed and paraffin-embedded human Tonsil tissue reacted with CD4 antibody (C-term) (Cat.#AP1496b), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of CEM cells using CD4 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CD4 Antibody (C-term) - Research Areas
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BACKGROUND
CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes (helper/inducer T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
REFERENCES
Garron,M.L., J. Mol. Biol. 375 (5), 1320-1328 (2008) Rychert,J., J. Acquir. Immune Defic. Syndr. 46 (3), 261-267 (2007)
