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PPP2R5A Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PPP2R5A Antibody (C-term) AP14971b
    PPP2R5A Antibody (C-term) (Cat. #AP14971b) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the PPP2R5A antibody detected the PPP2R5A protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q15172
Other Accession Q6PD03, NP_006234.1
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 56194 Da
Antigen Region 418-446 aa
Additional Information
Gene ID 5525
Other Names Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform, PP2A B subunit isoform B'-alpha, PP2A B subunit isoform B56-alpha, PP2A B subunit isoform PR61-alpha, PR61alpha, PP2A B subunit isoform R5-alpha, PPP2R5A
Target/Specificity This PPP2R5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 418-446 amino acids from the C-terminal region of human PPP2R5A.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPPP2R5A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPP2R5A
Function The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Cellular Location Cytoplasm. Nucleus. Chromosome, centromere. Note=From mitotic prophase to metaphase, localizes at the inner centromere between a pair of sister kinetochores. Decreased expression at the onset of anaphase
Tissue Location Widely expressed with the highest expression in heart and skeletal muscle
Research Areas
Citations (0)
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Background

The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B56 subfamily.

References

Flegg, C.P., et al. J. Biol. Chem. 285(24):18144-18154(2010)
Freeman, A.K., et al. Cell Cycle 9(4):736-747(2010)
Reece, K.M., et al. Biochem. Biophys. Res. Commun. 386(4):582-587(2009)
Li, H., et al. Mol. Cell. Biol. 29(3):919-928(2009)
Ruvolo, V.R., et al. J. Biol. Chem. 283(51):35474-35485(2008)

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$ 182.50
$ 70.00
Cat# AP14971b
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