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ATF5 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ATF5 Antibody (C-term) AP14991b
    All lanes : Anti-ATF5 Antibody (C-term) at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: human heart lysate Lane 4: mouse liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ATF5 Antibody (C-term) AP14991b
    ATF5 Antibody (C-term) (Cat. #AP14991b) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the ATF5 antibody detected the ATF5 protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9Y2D1
Other Accession NP_036200.2, NP_001180575.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 30674 Da
Antigen Region 188-216 aa
Additional Information
Gene ID 22809
Other Names Cyclic AMP-dependent transcription factor ATF-5, cAMP-dependent transcription factor ATF-5, Activating transcription factor 5, Transcription factor ATFx, ATF5, ATFX
Target/Specificity This ATF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-216 amino acids from the C-terminal region of human ATF5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATF5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATF5
Synonyms ATFX
Function Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4.
Cellular Location Cytoplasm. Nucleus {ECO:0000255|PROSITE-ProRule:PRU00978, ECO:0000269|PubMed:15358120}
Citations (0)

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Background

Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4.

References

Wei, Y., et al. J. Biochem. 148(2):171-178(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Tomppo, L., et al. Biol. Psychiatry 65(12):1055-1062(2009)
Li, G., et al. Mol. Cancer Res. 7(6):933-943(2009)
Uekusa, H., et al. Biochem. Biophys. Res. Commun. 380(3):673-678(2009)

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$ 295.00
$ 99.00
Cat# AP14991b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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