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Mouse TLR9 Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| AP1509d | DISCONTINED INQUIRE CLICK INQUIRE Add to cart | |||
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
Mouse TLR9 Antibody (C-term) - Product info | |
| Application | WB, IHC
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| Primary Accession | Q9EQU3 |
| Reactivity | Human, Mouse |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 116412 Da |
Mouse TLR9 Antibody (C-term) - Additional info | |
| Gene ID 81897 | |
| Other Names Tlr9; Toll-like receptor 9; CD_antigen=CD289; Flags: Precursor | |
| Target/Specificity This Mouse TLR9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 963~992 amino acids from the C-terminal region of mouse TLR9. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Mouse TLR9 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
Mouse TLR9 Antibody (C-term) - Protein Information | |
| Name Tlr9 | |
| Function Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response Plays a role in defense against systemic mouse cytomegalovirus infection. Controls lymphocyte response to Helicobacter infection | |
| Cellular Location Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endosome. Lysosome. Cytoplasmic vesicle, phagosome. Note=Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist | |
| Tissue Location Expressed in the basolateral region of gastric epithelial cells with high levels detected in antrum and body mucosa (at protein level). Detected in spleen and stomach at higher levels in C57BL/6 mice than BALB/C | |
Mouse TLR9 Antibody (C-term) - Related products
Mouse TLR9 Antibody (C-term) - Application data
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The mouse anti-TLR9 Pab (Cat. #AP1509d) is used in Western blot to detect TLR9 in mouse spleen tissue lysate.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Mouse TLR9 Antibody (R881) (Cat. #AP1509d) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the TLR9 antibody detected the TLR9 protein (arrow).
Mouse TLR9 Antibody (C-term) - Research Areas
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BACKGROUND
TLR9 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR9 is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.
REFERENCES
Chuang, T.H., et al., J. Leukoc. Biol. 71(3):538-544 (2002). Bauer, S., et al., Proc. Natl. Acad. Sci. U.S.A. 98(16):9237-9242 (2001). Hemmi, H., et al., Nature 408(6813):740-745 (2000).
