|Application ||WB, IHC-P, E|
|Calculated MW||28447 Da|
|Antigen Region||225-251 aa|
|Other Names||Dickkopf-related protein 2, Dickkopf-2, Dkk-2, hDkk-2, DKK2|
|Target/Specificity||This DKK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-251 amino acids from the C-terminal region of human DKK2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DKK2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).|
|Tissue Location||Expressed in heart, brain, skeletal muscle and lung|
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Provided below are standard protocols that you may find useful for product applications.
The 259-amino acid DKK2 protein, like DKK1, DKK3, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cys residues each. The second cys region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed expression of 4.0- and 4.5-kb DKK2 transcripts in heart, brain, skeletal muscle, and lung. Western blot analysis showed that DKK2 is secreted as a 15- to 17-kD protein. Functional analysis determined that DKK2 does not block Xenopus Wnt8 induction of a secondary axis in frog embryos.
Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003).
Brott, B.K., et al., Mol. Cell. Biol. 22(17):6100-6110 (2002).
Krupnik, V.E., et al., Gene 238(2):301-313 (1999).
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