|Application ||WB, IHC-P, E|
|Calculated MW||97916 Da|
|Antigen Region||820-851 aa|
|Other Names||Signal transducer and activator of transcription 2, p113, STAT2|
|Target/Specificity||This STAT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 820-851 amino acids from the C-terminal region of human STAT2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||STAT2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state (PubMed:9020188, PubMed:23391734). Acts as a regulator of mitochondrial fission by modulating the phosphorylation of DNM1L at 'Ser-616' and 'Ser-637' which activate and inactivate the GTPase activity of DNM1L respectively (PubMed:26122121).|
|Cellular Location||Cytoplasm. Nucleus. Note=Translocated into the nucleus upon activation by IFN-alpha/beta|
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Provided below are standard protocols that you may find useful for product applications.
STAT2 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus.
Rodriguez, J.J., et al., J. Virol. 77(21):11842-11845 (2003).
Gotoh, B., et al., J. Virol. 77(6):3360-3370 (2003).
Saleh, A.Z., et al., Biochemistry 41(37):11261-11268 (2002).
Rodriguez, J.J., et al., J. Virol. 76(22):11476-11483 (2002).
Stewart, M.D., et al., Biol. Reprod. 66(2):393-400 (2002).
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