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GJB2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GJB2 Antibody (C-term) AP1542a
    Western blot analysis of hGJB2-C218.Connexin (Cat. #AP1542a) in mouse brain tissue lysates (35ug/lane). GJB2 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - GJB2 Antibody (C-term) AP1542a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P29033
Other Accession A2VE67, P46691
Reactivity Human, Mouse
Predicted Bovine, Sheep
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 26215 Da
Antigen Region 204-226 aa
Additional Information
Gene ID 2706
Other Names Gap junction beta-2 protein, Connexin-26, Cx26, GJB2
Target/Specificity This GJB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 204-226 amino acids from the C-terminal region of human GJB2.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGJB2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GJB2
Function Structural component of gap junctions (PubMed:17551008, PubMed:19340074, PubMed:21094651, PubMed:26753910, PubMed:16849369, PubMed:19384972). Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannels, one from each cell membrane (PubMed:17551008, PubMed:19340074, PubMed:21094651, PubMed:26753910). Small molecules and ions diffuse from one cell to a neighboring cell via the central pore (PubMed:21094651, PubMed:16849369, PubMed:19384972).
Cellular Location Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. Note=Colocalizes with GJB4 at gap junction plaques in the cochlea. {ECO:0000250|UniProtKB:Q00977}
Research Areas
Citations (0)
citation

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Background

Gap junctions are conduits that allow the direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular metabolic and electrical communication. Gap junction channels consist of connexin protein subunits, which are encoded by a multigene family. GJBs (gap-junction proteins or connexins) play crucial functional roles associated with these channels. Immunohistochemical staining of human cochlear cells demonstrated high levels of GJB2 expression. Mutations in GJB2 are associated with genetically derived hearing impairments, including autosomal recessive nonsyndromic deafness.

References

Ohtsuka, A., et al., Hum. Genet. 112(4):329-333 (2003).
Yotsumoto, S., et al., Br. J. Dermatol. 148(4):649-653 (2003).
Uyguner, O., et al., Clin. Genet. 62(4):306-309 (2002).
Richard, G., et al., Am. J. Hum. Genet. 70(5):1341-1348 (2002).
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).

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$ 365.00
$ 140.00
Cat# AP1542a
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