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GJB6 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - GJB6 Antibody (N-term) AP1546a
    Western blot analysis of GJB6 (arrow) using rabbit polyclonal GJB6 Antibody (N-term) (Cat.#AP1546a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GJB6gene (Lane 2) (Origene Technologies).
  • IHC-P - GJB6 Antibody (N-term) AP1546a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • IHC-P - GJB6 Antibody (N-term) AP1546a
    Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with GJB6 Antibody (N-term) (Cat.#AP1546a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O95452
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 30387 Da
Antigen Region 87-117 aa
Additional Information
Gene ID 10804
Other Names Gap junction beta-6 protein, Connexin-30, Cx30, GJB6
Target/Specificity This GJB6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-117 amino acids from the N-terminal region of human GJB6.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGJB6 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GJB6
Function One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Cellular Location Cell membrane; Multi-pass membrane protein. Cell junction, gap junction
Research Areas
Citations (0)

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Gap junctions are conduits that allow the direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular metabolic and electrical communication. Gap junction channels consist of connexin protein subunits, which are encoded by a multigene family. GJBs (gap-junction proteins or connexins) play crucial functional roles associated with these channels. I Mutations in GJB2 are associated with genetically derived hearing impairments, including autosomal dominant, bilateral, middle to high frequency hearing loss.


Beltramello, M., et al., Biochem. Biophys. Res. Commun. 305(4):1024-1033 (2003).
Common, J.E., et al., Biochem. Biophys. Res. Commun. 298(5):651-656 (2002).
Smith, F.J., et al., J. Invest. Dermatol. 118(3):530-532 (2002).
del Castillo, I., et al., N. Engl. J. Med. 346(4):243-249 (2002).
Pallares-Ruiz, N., et al., Eur. J. Hum. Genet. 10(1):72-76 (2002).

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$ 295.00
$ 99.00
Cat# AP1546a
(40 western blots)
Availability: In Stock
Bulk Size
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