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Connexin 50 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P48165 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 48229 Da |
| Antigen Region | 99-130 aa |
| Gene ID | 2703 |
|---|---|
| Other Names | Gap junction alpha-8 protein, Connexin-50, Cx50, Lens fiber protein MP70, GJA8 |
| Target/Specificity | This Connexin 50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-130 amino acids from the N-terminal region of human Connexin 50. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Connexin 50 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GJA8 |
|---|---|
| Function | Structural component of eye lens gap junctions (PubMed:18006672, PubMed:19756179). Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannels, one from each cell membrane (By similarity). Small molecules and ions diffuse from one cell to a neighboring cell via the central pore (PubMed:18006672, PubMed:19756179). |
| Cellular Location | Cell membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:P55917}. Cell junction, gap junction |
| Tissue Location | Eye lens.. |
Provided below are standard protocols that you may find useful for product applications.
Background
GJA8 is a an integral membrane protein that belongs to the connexin family, alpha-type (group II) subfamily. One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. A connexon is composed of a hexamer of connexins. This particular connexin only forms junctional channels. GJA8 is expressed in the eye lens, and defects in GJA8 are the cause of zonular pulverulent cataract type 1 (CZP1), a form of autosomal dominant congenital cataract.
References
Shiels, A., et al., Am. J. Hum. Genet. 62(3):526-532 (1998).
Church, R.L., et al., Curr. Eye Res. 14(10):979-981 (1995).
Church, R.L., et al., Curr. Eye Res. 14(3):215-221 (1995).
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