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NCS1 Antibody (Center)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1551c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
NCS1 Antibody (Center) - Product info | |
| Application | WB, IHC, FC
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| Primary Accession | P62166 |
| Other Accession | Q91614, P62168, Q8BNY6, P62167, Q2V8Y7, NP_055101.2 |
| Reactivity | Human |
| Predicted | Bovine, Chicken, Mouse, Rat, Xenopus |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 21879 Da |
NCS1 Antibody (Center) - Additional info | |
| Gene ID 23413 | |
| Other Names NCS1; FLUP; FREQ; Neuronal calcium sensor 1; Frequenin homolog; Frequenin-like protein; Frequenin-like ubiquitous protein | |
| Target/Specificity This NCS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124~153 amino acids from the center region of human NCS1. | |
| Dilution WB~~1:50~100WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions NCS1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
NCS1 Antibody (Center) - Protein Information | |
| Name NCS1 | |
| Synonyms FLUP, FREQ | |
| Function Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity). Stimulates PI4KB kinase activity (By similarity). Involved in long-term synaptic plasticity through its interaction with PICK1 (By similarity). May also play a role in neuron differentiation through inhibition of the activity of N- type voltage-gated calcium channel (By similarity) | |
| Cellular Location Golgi apparatus, Golgi stack membrane; Peripheral membrane protein. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity). Cytoplasm, perinuclear region. Cell membrane; Peripheral membrane protein Note=Associated with Golgi stacks. Post-synaptic densities of dendrites, and in the pre-synaptic nerve terminal at neuromuscular junctions | |
NCS1 Antibody (Center) - Related products
AP14572a: FREQ Antibody (N-term)
AP1551c: NCS1 Antibody (Center)
DC01532: Human FREQ cDNA Clone
NCS1 Antibody (Center) - Application data
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Western blot analysis of NCS1 Antibody (Center) (Cat.# AP1551c) in MCF-7 cell line lysates (35ug/lane). NCS1 (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human breast carcinoma reacted with NCS1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of ZR-75-1 cells using NCS1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
NCS1 Antibody (Center) - Research Areas
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BACKGROUND
NCS1 is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. NCS1 regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. This protein is thought to be associated with secretory granules and may be involved in the regulation of neurosecretion.
REFERENCES
Koh, P.O., et al., Proc. Natl. Acad. Sci. U.S.A. 100(1):313-317 (2003). Bourne, Y., et al., J. Biol. Chem. 276(15):11949-11955 (2001). Burgoyne, R.D., et al., Biochem. J. 353 (Pt 1), 1-12 (2001).
