|Application ||WB, IHC-P, E|
|Calculated MW||23130 Da|
|Antigen Region||169-200 aa|
|Other Names||Recoverin, Cancer-associated retinopathy protein, Protein CAR, RCVRN, RCV1|
|Target/Specificity||This Recoverin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-200 amino acids from the C-terminal region of human Recoverin.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Recoverin Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Seems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin. May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent manner. The calcium-bound recoverin prolongs the photoresponse.|
|Tissue Location||Retina and pineal gland.|
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Provided below are standard protocols that you may find useful for product applications.
Recoverin belongs to a high-affinity calcium-binding family that includes neuronal calcium sensor-1, visinin-like proteins (VILIPs), guanylate cyclase-activating proteins (GCAPs), and Kv-channel interacting proteins (KchIPs). Features common to this family include four calcium-binding EF-hand domains, and an N-terminal myristoylation sequence. This family of proteins has been implicated in a broad range of cellular signaling functions, including phototransduction and neurotransmitter release, lipid metabolism, gene expression, and ion channel regulation. Myristoylation, the post-translational addition of a fatty acid tail, has been shown to have functional significance for other calcium-binding protein family members. Recoverin is subject to the posttranslational modification of myristoylation. Binding of calcium to recoverin elicits a change in conformation that exposes the buried hydrophobic myristoyl moiety to interaction with cell membranes and other cellular proteins.
Wiechmann, A., et al., Curr. Eye Res. 26(1):25-32 (2003). Matsubara, S., et al., Br. J. Cancer 74(9):1419-1422 (1996). Yamaji, Y., et al., Int. J. Cancer 65(5):671-676 (1996). McGinnis, J.F., et al., Mamm. Genome 4(1):43-45 (1993). Wiechmann, A.F., et al., Exp. Eye Res. 56(4):463-470 (1993).
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