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FAU Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - FAU Antibody (Center) AP1600c
    Western blot analysis of anti-FUBI Center Pab (AP1600c) in Hela and T47D cell line lysates. FUBI Center(arrow) was detected using the purified Pab.
    detail
  • IHC-P - FAU Antibody (Center) AP1600c
    Formalin-fixed and paraffin-embedded human colon carcinoma reacted with FUBI Antibody (Center)(Cat.#AP1600c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P35544
Other Accession P62864, P62863, P62862, P62861, P62860, P62866, NP_001988
Reactivity Human
Predicted Bovine, Hamster, Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Antigen Region 60-93 aa
Additional Information
Other Names Ubiquitin-like protein FUBI, FAU
Target/Specificity This FAU antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-93 amino acids from the center region of human FUBI.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFAU Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Citations (0)
citation

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Background

This gene is the cellular homolog of the fox sequence in the Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV). It encodes a fusion protein consisting of the ubiquitin-like protein fubi at the N terminus and ribosomal protein S30 at the C terminus. It has been proposed that the fusion protein is post-translationally processed to generate free fubi and free ribosomal protein S30. Fubi is a member of the ubiquitin family, and ribosomal protein S30 belongs to the S30E family of ribosomal proteins. Whereas the function of fubi is currently unknown, ribosomal protein S30 is a component of the 40S subunit of the cytoplasmic ribosome. Pseudogenes derived from this gene are present in the genome. Similar to ribosomal protein S30, ribosomal proteins S27a and L40 are synthesized as fusion proteins with ubiquitin.

References

Rossman, T.G., et al., Oncogene 22(12):1817-1821 (2003).
Kenmochi, N., et al., Genome Res. 8(5):509-523 (1998).
Vladimirov, S.N., et al., Eur. J. Biochem. 239(1):144-149 (1996).
Kas, K., et al., Genomics 17(2):387-392 (1993).
Michiels, L., et al., Oncogene 8(9):2537-2546 (1993).

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$ 182.50
$ 70.00
Cat# AP1600c
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