|Application ||WB, E|
|Other Accession||P46629, NP_065120.2|
|Calculated MW||23496 Da|
|Antigen Region||113-141 aa|
|Other Names||Ras-related protein Rab-25, CATX-8, RAB25, CATX8|
|Target/Specificity||This RAB25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-141 amino acids from the Central region of human RAB25.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RAB25 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia (PubMed:17925226). Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions (By similarity). May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis (By similarity).|
|Cellular Location||Cell membrane; Lipid-anchor; Cytoplasmic side. Cell projection, pseudopodium membrane. Cytoplasmic vesicle. Note=Colocalizes with integrin alpha-V/beta-1 in vesicles at the pseudopodial tips|
|Tissue Location||Expressed in ovarian epithelium (NOE) and breast tissue. Expressed in ovarian cancer; expression is increased relative to NOE cells. Expression in ovarian cancer is stage dependent, with stage III and stage IV showing higher levels than early stage cancers. Expressed in breast cancer; expression is increased relative to normal breast tissue|
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Provided below are standard protocols that you may find useful for product applications.
RAB proteins, such as RAB25, are members of the RAS superfamily of small GTPases that are involved in membrane trafficking. Members of the RAB11 subfamily, including RAB25, control the return of internalized membrane-associated moieties to the cell surface (Caswell et al., 2007 [PubMed 17925226]).[supplied by OMIM].
Cheng, J.M., et al. Int. J. Cancer 126(12):2799-2812(2010)
Nam, K.T., et al. J. Clin. Invest. 120(3):840-849(2010)
Caswell, P.T., et al. Dev. Cell 13(4):496-510(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Fan, Y., et al. Pathology 38(6):561-567(2006)
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