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NEIL2 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q969S2 |
| Other Accession | NP_001129220.1, NP_001129218.1, NP_659480.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 36826 Da |
| Antigen Region | 67-96 aa |
| Gene ID | 252969 |
|---|---|
| Other Names | Endonuclease 8-like 2, 322-, DNA glycosylase/AP lyase Neil2, DNA-(apurinic or apyrimidinic site) lyase Neil2, Endonuclease VIII-like 2, Nei homolog 2, NEH2, Nei-like protein 2, NEIL2 |
| Target/Specificity | This NEIL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-96 amino acids from the N-terminal region of human NEIL2. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NEIL2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NEIL2 |
|---|---|
| Function | Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Has DNA glycosylase activity towards 5- hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double-stranded DNA (DNA bubbles). Has low or no DNA glycosylase activity towards thymine glycol, 2-hydroxyadenine, hypoxanthine and 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. |
| Cellular Location | Nucleus. |
| Tissue Location | Detected in testis, skeletal muscle, heart, brain, placenta, lung, pancreas, kidney and liver |
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Provided below are standard protocols that you may find useful for product applications.
Background
NEIL2 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226])
References
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)
Monsees, G.M., et al. Breast Cancer Res. Treat. (2010) In press :
Grin, I.R., et al. Biochem. Biophys. Res. Commun. 394(1):100-105(2010)
Kinslow, C.J., et al. Genes Chromosomes Cancer 47(11):923-932(2008)
Zhai, X., et al. Clin. Cancer Res. 14(13):4345-4352(2008)
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