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NFIC Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - NFIC Antibody (Center) AP16052c
    All lanes : Anti-NFIC Antibody (Center) at 1:1000 dilution Lane 1: Hela whole cell lysates Lane 2: A549 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 56 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IF - NFIC Antibody (Center) AP16052c
    Fluorescent confocal image of Hela cell stained with NFIC Antibody (Center)(Cat#AP16052c). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFIC primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NFIC immunoreactivity is localized to nucleus significantly.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08651
Other Accession NP_995315.1, NP_005588.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 55675 Da
Antigen Region 180-208 aa
Additional Information
Gene ID 4782
Other Names Nuclear factor 1 C-type, NF1-C, Nuclear factor 1/C, CCAAT-box-binding transcription factor, CTF, Nuclear factor I/C, NF-I/C, NFI-C, TGGCA-binding protein, NFIC, NFI
Target/Specificity This NFIC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 180-208 amino acids from the Central region of human NFIC.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNFIC Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms NFI
Function Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Cellular Location Nucleus.
Research Areas
Citations (0)

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NFIC recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.


Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Nilsson, J., et al. Cancer Res. 70(5):2020-2029(2010)
Udelhoven, M., et al. J. Mol. Endocrinol. 44(2):99-113(2010)
Plasari, G., et al. Mol. Cell. Biol. 29(22):6006-6017(2009)
Riffel, A.K., et al. Mol. Pharmacol. 76(5):1104-1114(2009)

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$ 295.00
$ 99.00
Cat# AP16052c
(40 western blots)
Availability: In Stock
Bulk Size
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