CAPG Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | P40121 |
Other Accession | Q6AYC4, P24452, NP_001738.2 |
Reactivity | Human |
Predicted | Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 38499 Da |
Antigen Region | 181-209 aa |
Gene ID | 822 |
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Other Names | Macrophage-capping protein, Actin regulatory protein CAP-G, CAPG, AFCP, MCP |
Target/Specificity | This CAPG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 181-209 amino acids from the Central region of human CAPG. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | CAPG Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CAPG |
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Synonyms | AFCP, MCP |
Function | Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA. |
Cellular Location | Nucleus. Cytoplasm Melanosome. Cell projection, lamellipodium {ECO:0000250|UniProtKB:P24452}. Cell projection, ruffle {ECO:0000250|UniProtKB:P24452}. Note=In macrophages, may be predominantly cytoplasmic. Nuclear localization was observed in fibroblasts. In macrophages, present at the membrane-cytoplasm interface. In activated macrophages, concentrated in the ruffles of the leading lamellipodia. {ECO:0000250|UniProtKB:P24452} |
Tissue Location | Macrophages and macrophage-like cells. |
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Background
This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed, but have not been fully described. [provided by RefSeq].
References
Li, M.X., et al. Med. Oncol. 27(1):134-144(2010)
Burillo, E., et al. Atherosclerosis 207(1):32-37(2009)
Zhang, R., et al. Am. J. Respir. Cell Mol. Biol. 41(4):467-475(2009)
Hubert, T., et al. Biochem. Biophys. Res. Commun. 380(1):166-170(2009)
Chen, M., et al. J. Biol. Chem. 284(3):1484-1494(2009)
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